Protein Purification
Lectures and textbooks can cover the theoretical aspects of protein purification and laboratory classes can teach the practical techniques, but there are other topics which are difficult to learn by conventional methods. In order to purify any protein you need to know which separation techniques are likely to be most effective under the circumstances and, probably more important, which techniques are not. This knowledge cannot be picked up by following a fixed recipe for a class practical. It requires some thought and usually comes with experience, generally during postgraduate research.
Protein Purification is the latest version of the award-winning program which has been widely used in schools, colleges and universities since 1983. It aims to guide you through a simulation of some of the more commonly-used protein separation techniques and to let you experiment with the simulation. It starts off by letting you examine how a simple mixture of proteins behaves during gel filtration and ion-exchange chromatography and then goes on to allow the design and testing of full purification protocols using more complex mixtures of proteins.
It is assumed that you are familiar with the theoretical background to the most common separation techniques, enzyme assays etc. and that you understand the concept of the isoelectric point of proteins. The simulation models failure as accurately as success - so you need to be careful!
Standalone webapp versions of Protein Purification are available for Windows 2000/XP/Vista/7 and OS X Snow Leopard, Lion and Mountain Lion. A free online version is also available for desktops and laptops.
