| Diluting fractions | 1.0 | |
| Heat treatment | 1.0 | |
| Assay enzyme | 2.0 | |
| Ammonium sulfate fractionation | 2.0 | |
| 1D SDS PAGE | 3.0 | |
| 2D SDS PAGE | 5.0 | |
| Gel filtration | 5.0 | |
| Ion-exchange chromatography | 5.0 | |
| Hydrophobic interaction chromatography | 5.0 | |
| Affinity chromatography | 25.0 |
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